cu cpt22 Search Results


cu cpt22  (MedChemExpress)
94
MedChemExpress cu cpt22
PFFs activated primary microglia by binding to TLR1/2 or TLR4. Primary microglia were pretreated with <t>Cu-CPT22,</t> resatorvid, or E6446 for 12 h and then treated with PFFs for further 24 h. Levels of TNF α (a) and IL-1 β (b) were measured by ELISA assay. (c-f) Expressions of IRAK1, TRAF6, p-IKK α / β /IKK α / β ratio, and p-NF- κ B/NF- κ B ratio were measured by Western blot. (g) Translocations of NF- κ B were monitored by immunofluorescence staining. Scale bar = 15 μ m. The values were expressed as mean ± SEM. ∗∗∗ p < 0.001.
Cu Cpt22, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+cpt22/pmc06512043-34-0-11?v=MedChemExpress
Average 94 stars, based on 1 article reviews
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cu-cpt22  (MuseChem Chemicals)
N/A
CU CPT 22(cas# 1416324-85-0) is a selective toll-like receptor 1/2 (TLR1/2) inhibitor (IC50 = 0.58 μM), exhibits no activity at TLR2/TLR6, TLR3, TLR4 and TLR7 or a panel of 10 representative kinases. CU CPT 22
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cu cpt22  (Tocris)
94
Tocris cu cpt22
PFFs activated primary microglia by binding to TLR1/2 or TLR4. Primary microglia were pretreated with <t>Cu-CPT22,</t> resatorvid, or E6446 for 12 h and then treated with PFFs for further 24 h. Levels of TNF α (a) and IL-1 β (b) were measured by ELISA assay. (c-f) Expressions of IRAK1, TRAF6, p-IKK α / β /IKK α / β ratio, and p-NF- κ B/NF- κ B ratio were measured by Western blot. (g) Translocations of NF- κ B were monitored by immunofluorescence staining. Scale bar = 15 μ m. The values were expressed as mean ± SEM. ∗∗∗ p < 0.001.
Cu Cpt22, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+cpt22/pm28570013-279-28-29?v=Tocris
Average 94 stars, based on 1 article reviews
cu cpt22 - by Bioz Stars, 2026-08
94/100 stars
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tlr2 inhibition  (Selleck Chemicals)
93
Selleck Chemicals tlr2 inhibition
Fig. 4 The activation of <t>TLR2</t> is essential for inducing DCN+ macrophages by B.adolescentis. A The heatmap of differentially expressed TLRs genes in RNA-seq of BMDMs treated with B.adolescentis or vehicle (PBS). B The levels of differentially expressed TLRs genes in BMDMs treated with B. adolescentis were determined by qRT-PCR. C BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and DCN were tested by Western blot. D, E BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22. Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of Dcn was tested by qRT-PCR. F-I THP-1 cells were incubated with B. adolescentis or vehicle (PBS) for 24 h (H-G). THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22 (H, I). Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of DCN was tested by qRT-PCR. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 3 mg/kg Cu-CPT22 or vehicle (5% DMSO) were injected intraperitoneally to mice every two days. Tumor volume was recorded after 6 days. M The positive ratio of Ki67 in mice tumor tissue. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (B, G), ANOVA test (E, I, K, L, M)
Tlr2 Inhibition, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+cpt22/pm37464382-83-1-5?v=Selleck+Chemicals
Average 93 stars, based on 1 article reviews
tlr2 inhibition - by Bioz Stars, 2026-08
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cu-cpt22  (Merck KGaA)
90
Merck KGaA cu-cpt22
Fig. 4 The activation of <t>TLR2</t> is essential for inducing DCN+ macrophages by B.adolescentis. A The heatmap of differentially expressed TLRs genes in RNA-seq of BMDMs treated with B.adolescentis or vehicle (PBS). B The levels of differentially expressed TLRs genes in BMDMs treated with B. adolescentis were determined by qRT-PCR. C BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and DCN were tested by Western blot. D, E BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22. Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of Dcn was tested by qRT-PCR. F-I THP-1 cells were incubated with B. adolescentis or vehicle (PBS) for 24 h (H-G). THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22 (H, I). Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of DCN was tested by qRT-PCR. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 3 mg/kg Cu-CPT22 or vehicle (5% DMSO) were injected intraperitoneally to mice every two days. Tumor volume was recorded after 6 days. M The positive ratio of Ki67 in mice tumor tissue. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (B, G), ANOVA test (E, I, K, L, M)
Cu Cpt22, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+cpt22/pmc06481764-162-14-15?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
cu-cpt22 - by Bioz Stars, 2026-08
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cu cpt 22  (GlpBio Technology Inc)
90
GlpBio Technology Inc cu cpt 22
Fig. 4 The activation of <t>TLR2</t> is essential for inducing DCN+ macrophages by B.adolescentis. A The heatmap of differentially expressed TLRs genes in RNA-seq of BMDMs treated with B.adolescentis or vehicle (PBS). B The levels of differentially expressed TLRs genes in BMDMs treated with B. adolescentis were determined by qRT-PCR. C BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and DCN were tested by Western blot. D, E BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22. Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of Dcn was tested by qRT-PCR. F-I THP-1 cells were incubated with B. adolescentis or vehicle (PBS) for 24 h (H-G). THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22 (H, I). Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of DCN was tested by qRT-PCR. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 3 mg/kg Cu-CPT22 or vehicle (5% DMSO) were injected intraperitoneally to mice every two days. Tumor volume was recorded after 6 days. M The positive ratio of Ki67 in mice tumor tissue. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (B, G), ANOVA test (E, I, K, L, M)
Cu Cpt 22, supplied by GlpBio Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+cpt22/pm35659940-233-5-11?v=GlpBio+Technology+Inc
Average 90 stars, based on 1 article reviews
cu cpt 22 - by Bioz Stars, 2026-08
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tlr2 specific inhibitor cu cpt22  (Bomac Laboratories Limited)
86
Bomac Laboratories Limited tlr2 specific inhibitor cu cpt22
Fig. 4 The activation of <t>TLR2</t> is essential for inducing DCN+ macrophages by B.adolescentis. A The heatmap of differentially expressed TLRs genes in RNA-seq of BMDMs treated with B.adolescentis or vehicle (PBS). B The levels of differentially expressed TLRs genes in BMDMs treated with B. adolescentis were determined by qRT-PCR. C BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and DCN were tested by Western blot. D, E BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22. Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of Dcn was tested by qRT-PCR. F-I THP-1 cells were incubated with B. adolescentis or vehicle (PBS) for 24 h (H-G). THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22 (H, I). Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of DCN was tested by qRT-PCR. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 3 mg/kg Cu-CPT22 or vehicle (5% DMSO) were injected intraperitoneally to mice every two days. Tumor volume was recorded after 6 days. M The positive ratio of Ki67 in mice tumor tissue. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (B, G), ANOVA test (E, I, K, L, M)
Tlr2 Specific Inhibitor Cu Cpt22, supplied by Bomac Laboratories Limited, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cu+cpt22/pm41478530-77-45-39?v=Bomac+Laboratories+Limited
Average 86 stars, based on 1 article reviews
tlr2 specific inhibitor cu cpt22 - by Bioz Stars, 2026-08
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cu cpt 22  (Cayman Chemical)
N/A
CU CPT 22
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cu-cpt22  (TargetMol)
N/A
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cu cpt 22  (Aladdin Scientific Corporation)
N/A
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cu cpt 22  (BOC Sciences)
N/A
CU CPT 22 is a selective TLR1/2 inhibitor (IC50 = 0.58 μM).
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Image Search Results


PFFs activated primary microglia by binding to TLR1/2 or TLR4. Primary microglia were pretreated with Cu-CPT22, resatorvid, or E6446 for 12 h and then treated with PFFs for further 24 h. Levels of TNF α (a) and IL-1 β (b) were measured by ELISA assay. (c-f) Expressions of IRAK1, TRAF6, p-IKK α / β /IKK α / β ratio, and p-NF- κ B/NF- κ B ratio were measured by Western blot. (g) Translocations of NF- κ B were monitored by immunofluorescence staining. Scale bar = 15 μ m. The values were expressed as mean ± SEM. ∗∗∗ p < 0.001.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Triptolide Inhibits Preformed Fibril-Induced Microglial Activation by Targeting the MicroRNA155-5p/SHIP1 Pathway

doi: 10.1155/2019/6527638

Figure Lengend Snippet: PFFs activated primary microglia by binding to TLR1/2 or TLR4. Primary microglia were pretreated with Cu-CPT22, resatorvid, or E6446 for 12 h and then treated with PFFs for further 24 h. Levels of TNF α (a) and IL-1 β (b) were measured by ELISA assay. (c-f) Expressions of IRAK1, TRAF6, p-IKK α / β /IKK α / β ratio, and p-NF- κ B/NF- κ B ratio were measured by Western blot. (g) Translocations of NF- κ B were monitored by immunofluorescence staining. Scale bar = 15 μ m. The values were expressed as mean ± SEM. ∗∗∗ p < 0.001.

Article Snippet: Cu-CPT22, resatorvid, E6446, and 3 α -aminocholestane (3AC) were purchased from MedchemExpress (New Jersey, USA).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Staining

Fig. 4 The activation of TLR2 is essential for inducing DCN+ macrophages by B.adolescentis. A The heatmap of differentially expressed TLRs genes in RNA-seq of BMDMs treated with B.adolescentis or vehicle (PBS). B The levels of differentially expressed TLRs genes in BMDMs treated with B. adolescentis were determined by qRT-PCR. C BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and DCN were tested by Western blot. D, E BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22. Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of Dcn was tested by qRT-PCR. F-I THP-1 cells were incubated with B. adolescentis or vehicle (PBS) for 24 h (H-G). THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22 (H, I). Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of DCN was tested by qRT-PCR. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 3 mg/kg Cu-CPT22 or vehicle (5% DMSO) were injected intraperitoneally to mice every two days. Tumor volume was recorded after 6 days. M The positive ratio of Ki67 in mice tumor tissue. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (B, G), ANOVA test (E, I, K, L, M)

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Bifidobacterium adolescentis induces Decorin + macrophages via TLR2 to suppress colorectal carcinogenesis.

doi: 10.1186/s13046-023-02746-6

Figure Lengend Snippet: Fig. 4 The activation of TLR2 is essential for inducing DCN+ macrophages by B.adolescentis. A The heatmap of differentially expressed TLRs genes in RNA-seq of BMDMs treated with B.adolescentis or vehicle (PBS). B The levels of differentially expressed TLRs genes in BMDMs treated with B. adolescentis were determined by qRT-PCR. C BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and DCN were tested by Western blot. D, E BMDMs were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22. Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of Dcn was tested by qRT-PCR. F-I THP-1 cells were incubated with B. adolescentis or vehicle (PBS) for 24 h (H-G). THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 25 μM Cu-CPT22 (H, I). Protein levels of TLR2 and DCN were tested by Western blot and mRNA level of DCN was tested by qRT-PCR. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 3 mg/kg Cu-CPT22 or vehicle (5% DMSO) were injected intraperitoneally to mice every two days. Tumor volume was recorded after 6 days. M The positive ratio of Ki67 in mice tumor tissue. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (B, G), ANOVA test (E, I, K, L, M)

Article Snippet: For TLR2 inhibition, 3 mg/kg Cu-CPT22(Selleck Cat. #S8677) was injected intraperitoneally to mice every two days until sacrifice.

Techniques: Activation Assay, RNA Sequencing, Quantitative RT-PCR, Incubation, Western Blot, Injection

Fig. 5 B.adolescentis regulated DCN+ macrophages through TLR2/YAP axis. A, B BMDMs and THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and YAP were tested by Western blot. C Immunofluorescence assay of YAP distribution in BMDMs. BMDMs were stained with specific antibody against YAP (green), and the nuclei were counterstained with DAPI (blue). Scale bar, 10 μm. D, E Nuclear and cytoplasmic separation assay was performed in B.adolescentis-treated BMDMs and THP-1 cells, then the protein level of YAP in the nuclear and cytoplasm was detected by Western blot. F, G BMDMs and THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 1 μM verteporfin. Protein levels of TLR2, YAP and DCN were tested by Western blot. H, I YAP was overexpressed in BMDMs and THP-1 cells, and protein levels of YAP and DCN were tested by Western blot. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 50 mg/kg verteporfin or vehicle (5% DMSO) were injected intraperitoneally to mice every day. Tumor volume was recorded after 6 days. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001; ANOVA test (K, L)

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Bifidobacterium adolescentis induces Decorin + macrophages via TLR2 to suppress colorectal carcinogenesis.

doi: 10.1186/s13046-023-02746-6

Figure Lengend Snippet: Fig. 5 B.adolescentis regulated DCN+ macrophages through TLR2/YAP axis. A, B BMDMs and THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein levels of TLR2 and YAP were tested by Western blot. C Immunofluorescence assay of YAP distribution in BMDMs. BMDMs were stained with specific antibody against YAP (green), and the nuclei were counterstained with DAPI (blue). Scale bar, 10 μm. D, E Nuclear and cytoplasmic separation assay was performed in B.adolescentis-treated BMDMs and THP-1 cells, then the protein level of YAP in the nuclear and cytoplasm was detected by Western blot. F, G BMDMs and THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h with or without 1 μM verteporfin. Protein levels of TLR2, YAP and DCN were tested by Western blot. H, I YAP was overexpressed in BMDMs and THP-1 cells, and protein levels of YAP and DCN were tested by Western blot. J-L HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis or vehicle (PBS) for 24 h (n = 5 per group). From the beginning of tumor inoculation until sacrifice, 50 mg/kg verteporfin or vehicle (5% DMSO) were injected intraperitoneally to mice every day. Tumor volume was recorded after 6 days. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001; ANOVA test (K, L)

Article Snippet: For TLR2 inhibition, 3 mg/kg Cu-CPT22(Selleck Cat. #S8677) was injected intraperitoneally to mice every two days until sacrifice.

Techniques: Incubation, Western Blot, Immunofluorescence, Staining, Injection